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Fig. 6. Increased levels of ECE1 and Cx40 expression by CTB-induced pressure overloading between E3.5 and E6-6.5. (A) Video-captured image of an E3.5 embryo just after CTB (arrow, suture). (B) High power view of the heart region in A, showing a banding of the outflow tract without constriction at this stage. (C) E6.5 sham-operated heart. (D) E6.5 CTB (arrow) heart. (E) Relative expression levels of ECE1 and Cx40 normalized to GAPDH in individual ventricles of E6-6.5 control (white circle) and CTB (black circle) hearts. Line corresponds to best fit analysis of data points. Inset is an example of RT-PCR amplicons of GAPDH, ECE1 and Cx40 from control and CTB ventricles.