Fig. 3. Ttv affects HS GAG modifications in Dally and Dly. Total protein from third-instar larvae was analyzed by 4-20% gradient SDS-PAGE followed by western blotting with rabbit anti-Dly (Baeg et al., 2001) and rabbit anti-Dally (Lin and Perrimon, 1999) antibodies. In wild-type larvae, both Dly and Dally migrate as high molecular masses (M_r), characteristic of HS GAG-modified Dly and Dally (shown by arrows). In homozygous ttv^l(2)00681 larval extracts, the high M_r HS GAG-modified Dly is shifted to relative low M_r smear and the high M_r HS GAG-modified Dally is significantly reduced.