Fig. 6. T-box genes expression is down regulated in Zoep;ace mutants. (A-X,XX-XZ) All mount, in situ hybridization of embryos were taken from the progeny of double-heterozygous parents. The genotype (indicated top right) is inferred from either the phenotype (P-R,V-X,XX-XZ) or PCR-based genotyping. The embryonic stage is shown at bottom right (30%, 30% epiboly; sh, shield; 80%, 80% epiboly; 12s, 12 somites) and the probe (ntl, tbx6 and spt in blue) is indicated at bottom left. (A,G) Lateral views. (D,F,J,L,M-X,XX-XZ) Lateral views, dorsal to the right. (B,C,E,H,I,K) Animal pole views. (N,T) Arrowhead points to the prechordal plate, which is stained in N and lost in T. Note that the double-mutant phenotype is otherwise similar to wild-type. (Y-Z) PCR-based genotyping of embryos after in situ hybridization. Digested and nondigested (nd) PCR products were run on a 1.5% agarose gel.