Fig. 3. WOX2 and WOX8 mRNA expression patterns (brown) in the female gametophyte. In situ hybridization of unfertilized ovules. Sections showing the mature embryo sac as outlined in Fig. 2. (A) Hybridization with a WOX2 antisense probe. WOX2 mRNA is detected in the egg cell and the central cell, but not in the synergids. (B) Hybridization with a WOX8 antisense probe. WOX8 mRNA is also detected in the egg cell and the central cell, but not in the synergids. (C) Hybridization with a WOX8 sense probe. No staining was detected in any of the cells of the embryo sac. cc, central cell; ecn, egg cell nucleus; sc, synergid cells. Scale bars: 10 µm.