Fig. 2. Ectopic mouse Myf5 or MyoD expression in neural tube leads to skeletal muscle differentiation. Transverse sections of HH28 embryos transfected with either mouse Myf5 (A-C) or MyoD (D-H) were hybridised 4 days after electroporation with the DIG-antisense probes (blue) for mouse Myf5 (A-C) and MyoD (D-F) and then incubated with the MF20 antibody (brown) recognising all isoforms of myosin heavy chains or only incubated with the MF20 antibody (G,H). (G,H) Pictures of a different embryo from that of D-F. (B,C) Successive higher magnifications of the region below the DRG, framed in A. (E,F) Successive higher magnifications of the ventral part of the DRG framed in D. H is a higher magnification of the ventral part of the neural tube framed in G.