Fig. 6. Location of ectopic Notch receptor and ligand, 4 days after electroporation. Transverse sections from embryos electroporated with mouse MyoD (A-E) were hybridised 4 days after electroporation with the DIG-labelled antisense probe for mouse MyoD (C), Notch1 (A,B,D) and Serrate2 (E) followed by an immunohistochemistry using MF20 (A-D). The arrowhead in B shows the ectopic expression of Notch1 outside the MF20-positive cells. Arrows indicate the ectopic MF20-positive cells (B,D) and the muscle cells expressing the ligand Serrate2 (E).