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Fig. 6. Phylogenetic analysis of zinc-finger proteins that belong to the Sp class. A Tree-Puzzle analysis based on a Clustal W alignment places the T-Sp8 gene (boxed) together with its ortholog from Drosophila melanogaster (D-Sp1) close to the Sp7/osterix and the Sp8 (Bouwman and Philipsen, 2002) genes from vertebrates (Clustal W alignment; btd-Dm: buttonhead/Drosophila melanogaster was used as outgroup).