Fig. 7. Alk protein is mislocalised in mili^Alk mutant embryos. Stage 11 embryos stained with Fas3 (B,E,H) to mark all cells of the visceral mesoderm or Alk antibodies (green in A,D,G). The stainings are merged in C,F,I. In wild-type embryos (A-C), Alk is expressed in both the founder cells (f) and fusion-competent myoblasts (fcms) of the visceral mesoderm and localises at the cell membrane. (A-C). In jeb mutant embryos (D-F), Alk is also expressed at the membranes of all visceral cells. In mili^Alk mutants, the majority of the protein does not localise at the cell membranes but instead can be found in the cytoplasm (G-I). (J-M) In stage 11 wild-type embryos (J,K) Jeb (green in J-M) is taken up by the Alk-expressing cells of the visceral mesoderm (red in K,M), whereas in mili^Alk mutants (L,M) where the Alk protein is present but non-functional there is no co-localisation of the two proteins.