Fig. 2. Regulation of El and Noc at early stages. (A) dpp^Gal4-driven expression of the Wg-pathway inhibitor GSK3/Shaggy (Sgg) in an early third instar wing disc. Anti-El staining (purple) is reduced in the dpp expression domain (GFP, green). (B) dpp^Gal4-driven expression of Wg in a late second instar wing disc. El staining is ectopically induced in body wall regions in a non-autonomous manner close to the dpp expression domain. (C) dpp^Gal4-driven expression of the Wg-pathway inhibitor GSK3/Shaggy in an early third instar leg disc. El is reduced in the dpp expression domain (arrow). (D) dpp^Gal4-driven expression of the Dpp-pathway inhibitor Brinker (Brk) in an early third instar leg disc. El is reduced in the dpp expression domain (arrow).