Fig. 2. The pattern of cell migration is abnormal in the telencephalon of Pax6 mutants. (A) Experimental procedures for cell tracing in the telencephalon. DiI solution was injected into the rostral-most telencephalic wall of E12.5 embryos and whole embryos cultured for 48 hours. (B) Migration of the DiI-labeled cells in the telencephalon of wild type (WT, n=4) and Pax6 mutants (-/-, n=4). Compared to wild type, the mutant cells migrate caudally from the injection point. ^* P<0.05, t-test. (C,D) Wild-type (C) and mutant (D) embryos just after injection of DiI into the rostral-most telencephalon. Arrowhead indicates the injection points. (E,F) Migration patterns of labeled cells in the wild-type (E) and mutant (F) telencephalon. In the wild type, the majority of labeled cells are distributed at the rostral part of the telencephalon (C). In the Pax6 mutant, many labeled cells migrate caudally into the lateral part of the telencephalon (arrowheads in F). Scale bars: C,D, 1 mm; E,F, 200 µm.