Fig. 7. Misrouting of mitral cells is restored by transfection of Pax6 into the telencephalon of Pax6 mutants. (A) Expression vectors were electroporated into the rostral half of the mutant telencephalon. (B-F) Pax6-mutant embryos/brains transfected with either GFP alone (B) or GFP plus Pax6-expression vector (C-F). (B,C) lateral views of cultured brains. After 48 hours of WEC followed by 2 days of TOC, netrin G1-positive cells are distributed at the lateral surface of the telencephalon in the mutant introduced GFP-gene alone (arrowhead in B). By contrast, netrin G1-positive cells (arrowhead in C) are localized in the rostral-most telencephalon in the embryo electroporated with Pax6. (D-F) horizontal sections of the rostral part of the brains transfected with Pax6-expression vector. Nrp-positive cells are localized at the rostral-most telencephalon, which corresponds to the netrin G1-positive area (E). Pax6-immunopositive cells are distributed at the rostral part of the telencephalon after 2 days of TOC (F). (G) The location of the accumulation of netrin G1-positive cells in the mutant telencephalon after electroporation. tel, telencephalon; r, rostral; c, caudal. Scale bars: A, 500 µm; B,C, 1 mm; D-F, 50 µm.