Fig. 3. Tead2 binds to a Tead binding site within the Pax3 neural crest enhancer. An electrophoretic mobility shift assay is shown with Tead2 protein and an oligonucleotide derived from the NCE2 sequence. Tead2 binds to this site as seen in lane 1. Mutation of the Tead binding site abolishes binding (lane 2). When the known Tead co-factor YAP65 is added to the reaction, a supershift of the Tead2/DNA complex is observed (lane 3). Mutation of the Tead site abolishes the observed supershift (lane 4). All samples were run on the same EMSA gel.