Fig. 6. Mutation of the Tead2 binding site in the NCE2 enhancer abolishes transgenic expression in the neural tube and neural crest. (A) Transgenic embryo carrying a construct that includes a somite-specific Pax3 enhancer and the neural crest enhancers with a mutation in the Tead site of NCE2 is shown. No expression is evident in the dorsal neural tube or neural crest, while somite expression is maintained. (B) Transgenic embryo with the proximal 15 kb proximal Pax3 region including intact somites and neural crest enhancers demonstrates expression in both the somites and neural crest. (C) Whole-mount in situ hybridization demonstrating the wild-type Pax3 expression pattern. (D) Magnification of transgenic embryo shown in B.