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Fig. 4. Analysis of gene expression in Mili-/- mouse testes. The expression of stage-specific genes during spermatogenesis was analyzed by RT-PCR. G3PDH was used as the standard. Total testis RNA samples were prepared from 3-week-old and 12-week-old wild-type (+/+), heterozygous (+/-) and homozygous mutant (-/-) mice.