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Fig. 5. Expression of SYCP3, RAD51 and {gamma}-H2AX in Mili-/- spermatocytes. Anti-SYCP3 immunostaining (A-D), anti-RAD51 staining (E,H), anti-{gamma}-H2AX staining (K,N) and DAPI staining (F,I,L,O), and the merged images of RAD51 or {gamma}-H2AX and DAPI (G,J,M,P) in Mili+/- (upper panels of each pair) and Mili-/- (lower panels of each pare) testes. Arrows show the {gamma}-H2AX restricted to the sex bodies in early pachytene spermatocyte. All images except A and C were observed by confocal microscopy. Scale bars: 10 µm.