Fig. 6. No mid-pachytene spermatocyte in the Mili^-/- testes. Merged image of synaptonemal complexes stained with the B antiserum recognizing both COR1 and SYN1 (green) and DNA stained with DAPI (blue) (A-E) in mid-zygotene spermatocytes (A,D), early-pachytene spermatocytes (B,E) and mid-pachytene spermatocyte (C). The images of FITC-stained synaptonemal complexes and DAPI-stained nuclei were captured with L5 and A4 filters, respectively. The surface spreading samples were prepared from control testes (A-C) and the homozygous mutant testes (D,E). The micrographs of spermatogenic cell nuclei in Giemsa-stained preparations of heterozygous (F) and homozygous mutant (G) mice are shown. The arrows indicate mitotic metaphases of spermatogonia, and the arrowhead indicates the first meiotic metaphase of a primary spermatocyte.