Fig. 5. scraps (anillin) is required for the formation of microfilament
rings during cellularization. (A-D) Sagittal sections (A,C) and projections of
confocal sections of the cellularization front (B,D) of a
scrapsRS/scrapsPQ
mutant embryo shortly before (A,B) and after (C,D) the cellularization front
has passed the nuclear bases. Embryos were stained with antibody to myosin.
(A) The furrow canals of scraps mutant embryos during early
cellularization are only slightly abnormal. (B) Microfilament rings are not
present in scraps mutant embryos during early cellularization and the
basal lumens are less rounded than in wild-type embryos. Myosin is found in
aggregates scattered along the cellularization front (compare with
Fig. 2C). (C) The furrow canals
of scraps mutant embryos during late cellularization are collapsed
and lack a flask-like morphology. (D) Microfilament rings are not present in
scraps mutant embryos during late cellularization; the basal lumens
are angular and are less rounded than those during early cellularization in
scraps mutant embryos (compare with B). Myosin is found in aggregates
scattered along the cellularization front (compare with
Fig. 2G). Scale bar: 10
µm.