Fig. 6. Bottleneck protein co-localizes to the cellularization front with myosin. Cross-sections (A,C,E) and projections of confocal sections of the cellularization front (B,D,F) before the cellularization front has passed the nuclear bases of wild-type embryos. Embryos were stained with antibodies to Bnk and myosin, and images have been arranged to show Bnk protein (A,B), Myosin (C,D), and merged images of both Bnk and myosin (E,F). (A,B) Bnk is expressed along the entire furrow canal and in microfilament rings during early cellularization. (C,D) Myosin is expressed basal-laterally in the furrow canal and in microfilament rings. (E,F) Bnk and myosin localization overlaps in microfilament rings and overlaps basal-laterally in the furrow canal, but Bnk localization extends farther apically. Scale bar: 10 µm.