Fig. 7. Phenotypes of bnk and scraps are co-expressed.
Projections of confocal sections of the cellularization front (A-C) and
sagittal sections (D-F) of the same bnk mutant (A,D),
scrapsRS/scrapsPQ
mutant (B,E) and scrapsRS/scrapsPQ; bnk
double-mutant (C,F) embryos. Embryos were stained with antibody to myosin
(A-F) and with Hoechst dye (D-F). (A) Microfilament rings are hypercontracted
in bnk mutant embryos; some nuclei are constricted into dumbbell
shapes by the hyperconstricted microfilament rings and carried out of the
periphery by the cellularization front in bnk embryos (D). (B)
scraps mutant embryos showing the absence of microfilament rings,
angular basal lumens and a normal nuclear morphology (E). (C) Microfilament
rings are not formed in scraps; bnk mutant embryos, but the
cellularization front still exhibits increased contraction and large gaps in
the microfilament network. (F) Despite the absence of microfilament rings in
scraps; bnk mutant embryos, some nuclei are constricted into dumbbell
shapes by the contracted microfilaments and carried out of the periphery.
Scale bar: 10 µm.