Fig. 6. Hes5 expression is reduced in Math1^{ß-gal/ß-gal} CGC. Semi-quantitative RT-PCR analysis of Hes1, Hes5 and ß-actin expression in E14.5 rhombic lip (R.L.) tissue, and after 3 and 6 days (3 DIV and 6 DIV, respectively) in culture. Hes5 expression is greatly reduced in Math1^{ß-gal/ß-gal} (Null) compared with Math1^+/+ (WT). In contrast, Hes1 expression is not significantly altered between Math1^+/+ and Math1^{ß-gal/ß-gal} cells. The ß-Actin control indicates similar level of starting material. + and - indicate the presence and absence of reverse transcriptase, respectively.