Fig. 1. Effects of disrupting Wnt8 function. (A-F) Lateral views of live embryos at 30 hpf. wnt8 morphant (A), moderately affected dkk1-injected wild-type embryo (B) and severely affected dkk1-injected wild-type embryo lacking hindbrain and otic tissue (C). Enlarged view otic vesicles of wnt8 morphant (D), moderately affected dkk1-injected wild-type embryo (E) and wild-type embryo (F). (G,H) Lateral views of pax8 expression at tailbud stage in a wild-type embryo (G) and wnt8 morphant lacking the otic domain (H). (I-K) Dorsal views of pax8 expression at six-somite stage in a wild-type embryo (I), severely affected dkk1-injected embryo (J) and Dfw8 homozygote (K). Arrowheads indicate the preotic region. Abbreviation: mhb, midbrain-hindbrain border. (A-F) Anterior is to the left and dorsal upward. (G,H) Lateral views with anterior upward. (I-K) Dorsal views with anterior upward. Scale bars: A-C, 150 µm; D-F, 30 µm; G-K, 200 µm.