Fig. 5. Specificity of the ATG morpholino-induced phenotype. (A) Dose dependency of the observed phenotype. (B) Injection of up to 25 ng 4 bp mismatch morpholino does not cause any mutant phenotype. (C,D) Partial rescue of the morphant phenotype by co-injection of dfna5 DNA. Dorsal (C, anterior towards the top) and lateral (D, anterior towards the right) view of one d5 larva injected with 25 ng dfna5 ATG-MO and 70 pg dfna5 pCS2+. Left ear (C) and jaw (D) show malformations typical for injections of relatively high doses of ATG-MO, whereas the right ear is rescued and shows no morphological abnormalities. The anterior and posterior columns are properly fused, which is never observed in larvae injected with 25 ng ATG-MO only. Scale bar: 130 µm in B,C; 200 µm in D.