Fig. 6. Histological analysis of the inner ear epithelial columns in dfna5 morphants. Cross-sections of lateral semicircular canals at day 6 of wild-type (A,C) and dfna5 GT-MO injected (B,D) embryos. (A,B) Toluidine Blue staining of 5 µm sections, indicating a loss of the monolayer of the epithelium (A,B, arrow) and the basal lamina (A,B, arrowheads) in morphants (B) compared with wild type (A). (C,D) Structure of the basal lamina in TEM cross-sections. (C) The wild-type lamina has tightly packed, parallel fibers. (D) dfna5 morphant basal lamina is disrupted with loose, disorganized fibers. Scale bar: 20 µm in A,B; 1 µm in C,D.