Fig. 1. Effect of tws mutation on Wg, DV-Vg and Ct expression patterns. (A-C) Tws expression levels (detected by anti-Tws antibody staining) in wild-type (A), tws^P/+ (B) and tws^P/tws^P (C) wing discs. Homozygous mutants barely have any gene product. (D,E) Wild-type (D) and tws^P/tws^P (E) wing discs stained for Ci, which is expressed only in the anterior compartment. Note complete mirror-symmetry duplication in tws^P/tws^P disc as reported by Uemura et al. (Uemura et al., 1993). (F,G) Wild-type (F) and tws^P/tws^P (G) wing discs stained for Wg. (H,I) Higher magnification images of wild-type (H) and tws^P/tws^P (I) wing discs showing Wg expression in the DV boundary in more detail. Note that Wg levels in the DV boundary of tws^P/tws^P discs are marginally lower, while the expression pattern itself is unchanged compared with wild type. (J,K) Wild-type (J) and tws^P/tws^P (K) wing discs stained for vg-BE. There is no change in the expression pattern of vg-BE between normal and mutant discs. (L,M) Wild type (L) and tws^P/tws^P (M) wing discs stained for Ct. Note reduction in Ct expression levels in the mutant disc.