Fig. 3. mio affects meiotic progression. Wild-type (A) and mio mutant germaria (B) stained with C(3)G (red) and Orb (green). Magnified C(3)G staining of adjacent cysts are shown in small boxes. As is observed in wild type, in mio mutants fully developed SCs are formed in two to four cells in region-2a cyst. However, in mio cysts more than four cells enter the meiotic cycle and construct SC (see region-2a cyst traced with white line). (C,D) Anterior regions of wild type (C) and mio mutant germaria (D). Boxes indicate magnified regions of adjacent cysts stained with Hoechst (blue) and C(3)G (red). (C) Upper two cysts traced with white line are in early region 2a, determined by the absence of Orb expression. In wild type, early region-2a cysts sometimes show an early zygotene-like SC, with two to ten dots per nucleus observed in one or two cystocyte within a cyst. (D) In mio mutants, more extensive SCs are observed in early region 2a, which include numerous dots and short thread-like SCs that are often present in more than two cells per cyst.