Fig. 5. Identification of the mio gene (A) The physical map of the 23C-D cytogenetic interval. Black boxes indicate the regions that are deleted in each deficiency. Light hatched boxes are the regions of uncertainty. The mio (CG7074) ORF is shown as black box with arrow indicating the direction of transcription. The other 10 ORFs in the defined 50 kb interval are depicted as grey arrows. The mio gene is composed of seven exons. (B) The mio gene encodes a single transcript. Northern blot of total RNA from wild-type ovaries, probed with a mio cDNA. (C) The Mio protein contains an N-terminal domain with four WD40 repeats (grey box) and a RING/PHD finger-like domain near the C terminus (black box). An alignment of the four WD40 repeats and the RING/PHD finger-like domain of Mio, human FLJ20323, mouse BC20002, zebrafish BC047198, fission yeast SPAC630.02 and budding yeast YBL104c. The locations of the mio¹ and mio² truncations are indicated (arrowhead). Both yeast homologs lack the last WD40 repeat, while the mammalian homologs contain six WD40 repeats, two more than are found in Drosophila. All homologs except YBL104c contain a RING/PHD finger-like domain. (YBL104c shares only part of the conserved domain.) Black bar above the structure shows the region used as antigen. Stars indicate amino acid conservation between mio homologs and the indicated motifs. The consensus for the RING finger and PHD finger motifs are shown, respectively, above and below the mio alignments.