Fig. 6. Mio localizes to oocyte nuclei. (A) Western blot analysis of ovarian protein extracts. The Mio antibody recognizes a single band that coincides with the predicted molecular size of Mio, 98.6 kDa in extract from wild type (WT) and the Mio overexpression line (UASp-mio). Additionally, the antibody recognizes a slightly smaller protein (asterisk) in mio² ovarian extracts. mio² is predicted to encode a truncated Mio protein of 92.6 kDa. The last lane is a higher contrast picture of the mio² extract. (B) A wild-type ovariole stained with Mio. (C) Faint Mio signal is detected in two cells of a cyst in region 2a. The pattern is often not symmetric, with one nucleus (large arrow) frequently having a brighter signal. In region 2b and region 3, the staining is restricted to a single cell and dramatically increases in intensity (arrows). (D) C(3)G localization in the same germarium as depicted in C. Small box denotes magnified region-2b nucleus stained with Mio (red) and C(3)G (green). Arrows and arrowheads indicate Mio-positive cells in B and C(3)G-positive cells in D. E is a diagram showing the stages and localization of Mio (red) and C(3)G (green) in the germarium depicted in C and D. The Mio protein colocalizes to the nuclei that stain most intensely with C(3)G in region 2a (large arrowheads and arrows). However, the overlay of a region 2b nucleus demonstrates that the Mio protein has a different distribution than the SC component C(3)G (small box).