Fig. 7. Mutations in mei-W68 suppress the mio mutant phenotype. Ovarioles from mio² (A) and a mio², mei-W68¹ double mutant (B), stained with Hoechst (blue) and Orb (red). Unlike the single mutant, oocytes from double mutants form normal karyosomes (arrows) and accumulate Orb protein beginning in region 2a (arrowheads). Although most mio², mei-W68¹ egg chambers develop normally, some abnormalities are observed, such as the mispositioning of the oocyte (see third egg chamber in B where the oocyte is located in the center, rather than the posterior, of the egg chamber).