Fig. 4. Molecular characterization of JAG. (A) Genomic structure. Small vertical arrows indicate borders of restriction fragment used for recapitulation (see B). Triangles mark T-DNA insertions in jag-1 and jag-4. (B) Inflorescence of an intermediate line that recapitulates the jag-5D phenotype. (C) Confocal image of epidermal cells from the pedicel of a floral primordium transformed with a construct in which a JAG:GFP fusion protein is expressed under control of the AP1 promoter. (D) Alignment of predicted amino-acid sequences of JAG, JGL, OsJAG and SUP. Periods indicate residues that are identical or similar in at least two sequences, and small asterisks indicate residues that are identical in all sequences. Beneath the zinc-finger domain, pink arrows indicate residues that determine DNA-binding specificity.