Fig. 1. The GMC-1 division pattern in miti^P embryos. (A-F) Embryos stained for Eve; (G,H) embryos stained for Eve (red) and Zfh1 (green; yellow indicates co-localization); (I,J) embryos stained for Eve (red) and 22C10 (green). Embryos in A-H were analyzed by light microscopy, those in I and J were analyzed by confocal microscopy. Anterior end is up, vertical lines indicate the midline. Arrow indicates an RP2, small arrow indicates a sib. (A,B) Wild-type embryos, note the smaller sib (with fading Eve expression) and the larger RP2 (A); by 13 hours of age, the sib loses Eve expression and is no longer visible (B). (C-F) miti^P embryos. Multi-cell clusters are seen in the younger stage embryo (C, arrowheads), whereas in older stage miti^P embryos (D-F) two to three RP2s were observed per hemisegment. (E) Three RP2s and two sibs can be seen (sibs are barely visible). (G) Wild-type embryo stained for Eve and Zfh1. Note that the RP2 expresses Zfh1 at high levels. (H) miti^P embryo, all three RP2s co-express Eve and Zfh1. (I,J) Wild-type (I) and miti^P (J) embryos double stained for Eve and 22C10. The two Eve-positive RP2s also express 22C10 and project normally.