Fig. 2. The GMC-1 adopts a reiterating division pattern in embryos overexpressing miti or nub. Embryos were stained for Eve (red) and Zfh1 (green). Only merged images are shown, yellow indicates co-expression. The line drawings (C,E,G,I) are interpretations of the data from panels A,B,D,F,H. The boxed area in each line drawing indicates cells observed in the corresponding panels. (A,B) Wild-type embryos. The GMC-1 has terminally divided to generate a sib (Eve positive, Zfh1 negative) and an RP2 (Eve and Zfh1 positive) with no additional cells formed. (D,F) miti transgenic embryos in which the gene was induced at 6.75 hours of development for 30 minutes. At the 7.5-hour stage, the GMC-1 appears to have self-renewed (larger of the two cells) and generated an RP2 (D) or sib (F). By the 8.5-hour stage, the GMC-1 is dividing to generate a sib, an RP2 has already been generated (H). (J) An 10.5-hour stage miti^P embryo in which the transgene was induced at 6.75 hours of development for 90 minutes. A multi-cell cluster of several RP2s, one sib and a GMC-1 can be seen.