Fig. 5. Cyclin E protein is upregulated in late GMC-1 in embryos overexpressing miti. Embryos are double stained for CycE (green) and Eve (red). Only CycE and merged images of the two are shown. Arrowhead indicates a GMC-1, arrow indicates an RP2, small arrow indicates a sib. Only hemisegments are shown. (A,B) A late GMC-1 in a wild-type embryo (7.25 hours old) with very little CycE. (C,D) A newly formed RP2 and a sib in a wild-type embryo (7.75 hours old) with undetectable levels of CycE. (E,F) A late GMC-1 in a hs-miti embryo (7.25 hours old) without any induction. (G,H) A newly formed RP2 and a sib in a hs-miti embryo (7.75 hours old) without any induction. (I,J) A late GMC-1 in a hs-miti embryo induced for 20 minutes. Note the high levels of CycE. (K,L) A newly formed larger cell (presumably the self-renewed GMC-1, see text) and a sib in a hs-miti embryo where the transgene was induced for 20 minutes at the GMC-1 stage. The larger cell has high levels of CycE; CycE is undetectable in the smaller cell. (M,N) Two sibs and an RP2 in a hs-miti embryo (9.5 hours old) in which the transgene was induced for 20 minutes at the GMC-1 stage. (O,P) Three RP2 in a hsmiti embryo (9 hours old) in which the transgene was induced for 20 minutes at the GMC-1 stage. In panels M-P, CycE has been downregulated after the additional round of division. Scale bar: 7.5 µm.