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Fig. 6. The upregulation of CycE induces GMCs to undergo a self-renewing asymmetric division. Embryos in panels A-F are stained for Eve (red). Embryos in panels G-L are stained for Eve (red) and Zfh1 (green); co-localization of Eve and Zfh1 is indicated by yellow. Embryos in panels M and N are stained for Eve (green) and Vnd (red); yellow indicates co-localization, only the merged images are shown. Arrow indicates an RP2, small arrow indicates a sib, arrow with a star indicates a multi-cell cluster, arrowhead indicates an aCC, small arrowhead indicates a pCC, arrowhead with a star indicates friend of pCC (fpCC). (A-C) ~9-hour-old UAS-CycE; ftz-GAL4 embryos showing the 3-cell phenotypes with two sibs and an RP2 (A,B) or two RP2s and a sib (C). (D) A ~9-hour-old hs-CycE embryo in which the transgene was induced for 25 minutes at the GMC-1 stage. Note the 3-cell phenotype (2 sibs and an RP2). (E,F) A ~10.5-hour-old hs-CycE embryo in which the transgene was induced for 90 minutes at the GMC-1 stage. (G) ~9-hour-old wild-type embryo. (H,I) ~9-hour-old hs-CycE embryos in which the embryos were heat shocked for 30 minutes at ~6.75 hours of age. The GMC-1 has self-renewed once to generate either a sib (H) or an RP2 (I), and then terminally divided to generate an RP2 and a sib. (J) Wild-type embryo, showing an aCC (Eve and Zfh1 positive), a pCC (only Eve positive) and a fpCC (Eve and Zfh1 positive). (K,L) ~9-hour-old hs-CycE embryos in which the embryos were heat shocked for 30 minutes at ~6 hours of age. The GMC-1 has self-renewed to generate either two pCCs and an aCC (K) or two aCCs and a pCC (L). fpCC is formed later than aCC/pCC, therefore this particular induction timing does not affect its division pattern; a later induction affects fpCC as well (data not shown). (M) Wild-type embryo, showing an aCC (only Eve-positive), a pCC (Eve and Vnd positive) and a fpCC (Eve-positive). (N) ~9-hour-old hs-CycE embryos in which the embryos were heat shocked for 30 minutes at ~6 hours of age. The GMC-1 has self-renewed to generate two pCCs and an aCC.