Fig. 7. Self-renewing asymmetric division in ago mutant embryos. (A-C) Embryos stained for Eve. (D-I) Merged images of embryos stained for Eve (red) and CycE (green). Arrow indicates an RP2, small arrow indicates a sib, arrowhead indicates a GMC-1. Vertical lines mark the midline. (A) An 9.5-hour-old ago mutant embryo. The hemisegment on the left has the 3-cell phenotype (two sibs and one RP2). (B,C) An 11-hour-old ago mutant embryo with the same segment shown in two different focal planes. The hemisegment on the left has the 3-cell phenotype, two RP2s (B) and one sib with fading Eve expression (C). (D,E) A late GMC-1 in an ago mutant embryo (7.25 hours old) with high levels of CycE protein. (F,G) An 7.75-hour-old ago mutant embryo in which the GMC-1 appears to have nearly completed its division to generate two cells of more or less equal sizes. One of these two cells, presumably the self-renewed GMC-1, has high levels of CycE and the other cell, presumably the RP2, has very low levels of CycE. (H,I) A hemisegment showing a 3-cell phenotype in an 9-hour-old ago mutant embryo. All three cells have undetectable levels of CycE. Scale bars: 10µm for A-C; 7.5µm for D-I.