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Fig. 3. Aadc and Pitx3 expression in the mesencephalic neuronal field at E12.5. (A) Aadc expression in a sagittal section showing the mesencephalic dopaminergic (mesDA) field and additional positive neurons located dorsally in the midbrain and serotonergic neurons positioned caudal to the midbrain/hindbrain border (MHB). (B) Tyrosine hydroxylase (Th) expression in an adjacent section to A. (C) Schematic representation of a sagittal section showing the position of the coronal sections shown in D-I. (D-F) Aadc expression in coronal sections at three different positions as indicated in C. (G-H) Pitx3 expression in adjacent sections to D-F. Aq, aqueduct.