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Fig. 7. Retrograde tracing in the mesencephalic dopaminergic (mesDA) system and double labeling by Pitx3 immunohistochemistry. (A) Retrograde tracing in the control brain. The left two panels (FG) show the fluorogold label in the traced neurons in the mesDA system. The middle two panels (FG/Pitx3) show the double labeling. The arrow indicates one of the double labeled mesDA neurons. The right two panels (Pitx3) show the labeling by Pitx3 alone. The lower two panels show the injection site (right) and a schematic representation indicating the injection site (arrows). (B) Retrograde tracing in the ak mutant brain. The left panel represents the injection position. The right panel shows a neuron (arrow) in the VTA region that was traced by injection in the dorsal striatum.