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Fig. 1. Endogenous expression of Gata, Pet1 and Lmx1b. Whole-mount in situ hybridization of chick hindbrain showing expression of (A) Gata3 at HH18-19, (B) Pet1 at HH20-21, (C) Lmx1b at HH21-22, (D) Gata3 (blue), (E) Pet1 (blue), and (F) Lmx1b (blue) with Nkx2.2 (red) at HH24. The midbrain (MB), mid-hindbrain boundary (MHB, arrow), and the 5-HT progenitors (5-Htpr, arrowhead) are marked; the boundary between rhombomere1 (r1) and r2 is indicated by a horizontal black line.