Fig. 1. Generation of K14-BMP4 and K14-noggin transgenic mice. (A) Transgenic constructs as diagrammed were injected into one-cell stage Fvb embryos to generate founders. (B,D) Southern blot analyses of genomic DNA from mouse tails shows the BMP4 and noggin transgenic bands in the respective transgenic founders. (C,E) Western blot analyses of lysates from back skin of one-month-old mice shows that BMP4 and noggin proteins are overexpressed in the skin compared with wild-type (wt) littermates. (F-I) Whole-mount in situ hybridization of E12.5 mouse embryos shows that the Bmp4 (G) and noggin (I) transgenes are overexpressed in the whisker pad (arrows) of the corresponding transgenic embryos compared with wild-type littermates (F,H).