Fig. 9. Neurotrophin transcript expression in the mystacial pads of K14-noggin mice. Expression of neurotrophin mRNAs was analyzed by quantitative RT-PCR performed with mRNA extracted from the mystacial pads of E14.5 and postnatal day 2 (P2) K14-noggin and wild-type littermates. Shown are two curves each for transgenic (blue) and wild-type (red) tissues. At E14.5 there were no changes in NGF, NT3 or GDNF transcripts, while BDNF transcripts were slightly increased about twofold in the K14-noggin animals. At PN2 there were no changes in transcript levels of BDNF, NT3 or GDNF, but there was a small (fourfold) increase in NGF transcripts in the K14-noggin animals.