Fig. 7. Average loss of SerT activity between genotypes. Error bars represent the standard deviation of the mean. Genotypes were scored for the percent of hemisegments negative for SerT activity/total hemisegments per VNC. n represents the number of total hemisegments scored. At least 10 cords were averaged for each genotype. Wild-type cords have 0% loss of SerT. (a,b) P<0.001 (One-way ANOVA, Tukey comparison), showing that SerT activity is rescued by (a) Eg gain of function in an robo2 loss of function and (b) Robo2 gain of function in an eg hypomorph. No significant difference (ns, P>0.05) was found between robo2 loss of function and the double robo2 loss of function; eg^Mz360 mutants. robo2 loss of function score represents data from both robo2^x123 and robo2^x135 alleles, as phenotypes were indistinguishable.