Fig. 1. Real-time PCR confirming the expression changes of subsets of genes in Brn3b^-/- retina. The x-axis is relative fluorescence units (RFU), indicating PCR product accumulation, and the y-axis indicates PCR cycles. Wild type is red and Brn3b^-/- is green. ß-actin serves as an RNA input control. Syntg4 is synaptotagmin 4. The accumulation of fluorescence for Brn3b in the Brn3b^-/- sample near the end of the reaction is due to nonspecific amplification, as confirmed by the melting curve and agarose gel electrophoresis (data not shown). Fold changes obtained by real-time PCR are shown in Table 1.