(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. The sonic hedgehog pathway is disrupted in Brn3b-/- retinas. (A) Real-time RT-PCR analysis of genes belonging to the sonic hedgehog pathway. Red is for wild-type and green is for Brn3b-/-. ß-actin was used as RNA input control. Three genes, sonic hedgehog (Shh), Gli1 and patched 2 (Ptch2), were significantly downregulated in Brn3b-/- retina, while other genes showed no change. (B) In situ hybridization of Shh, Gli1 and Smo on E14.5 Brn3b heterozygous (+/-) and null (-/-) sections. Gli1 and Smo were expressed in progenitor cells and Shh in RGCs. Gli1 and Shh were downregulated in Brn3b-/- retinas, while Smo showed no change.