Fig. 2. Expression of p53, p63 and p73 proteins in oligodendrocyte lineage cells. Purified P7 rat OPCs were cultured in PDGF without TH and RA for 10 days. They were then cultured for 3 or 5 days in either PDGF alone or PDGF and TH to induce differentiation. (A-C) After 3 days, the cells were fixed and stained by immunofluorescence for p53 (A), p63 (B) or p73 (C). (D) Cells in PDGF and TH for 3 or 5 days were fixed and double-stained for both GC and p73. In all cases, nuclei were counterstained with bisbenzamide. Scale bar: 20 µm.