Fig. 6. Alterations in Hox gene expression in msd::Dll1^dn transgenic mice. In situ hybridisation on sections of paraffin-embedded day 12.5 embryos using Digoxigenin-labeled antisense riboprobes. Hoxb6 and Hoxc5 transcripts were detected in prevertebra (pv) 7 and subsequent posterior prevertebrae in wild type (A,A',D,D'), whereas anterior-most expression in transgenic embryos was limited to pv8 (B,B',E,E'). In Dll1^lacZ embryos Hoxb6 expression was detected in pv6 (C,C'). Arrowheads indicate the anterior-most pv with detected Hox gene expression.