Fig. 8. Shifted position of anterior limb buds in mice with impaired cyclic Lfng expression. Whole-mount in situ hybridisation with myogenin antisense riboprobes revealed a reduced number of segmental units anterior to the forelimb bud in LfngHA3, Lfng^lacZ/lacZ and Dll1^lacZ/lacZ 10.5 day embryos. In wild-type (A) and homozygous msd::Dll1^dn embryos (B) seven myotomes were detected anterior to the fore limb bud. Transgenic msd::LfngHA embryo (C) with six segments anterior to the forelimb bud, and homozygous mutant Lfng^lacZ/lacZ (D) and Dll1^lacZ/lacZ (E) embryos with five segments anterior to the forelimb bud, respectively. (G,G') In situ hybridisation on a day 12.5 Lfng^lacZ/lacZ embryo section showing anterior-most expression of Hoxb6 between pv4 and pv5 in contrast to pv7 in wild type (F,F'). Brackets in F,G indicate the extent of the first five pv.