Fig. 2. Induction of OLPs by the Hedgehog agonist SHHAg1.2.Mouse E13.5 neocortical neuroepithelial cells were cultured in the presence or absence of SHHAg for 4 DIV. The cultures were fixed and immunolabelled with polyclonal anti-NG2. (A) Control cultures lacked NG2 immunoreactivity. (B) Numerous NG2-positive cells developed in the presence of 100 nM SHHAg1.2. (C) The dose-response curve shows induction of NG2-positive cells at a half-maximal concentration of SHHAg1.2 of 25 nM.