Fig. 3. Loss of canonical Wnt signaling does not alter Hu expression. (A,B) Foxd3-positive neural crest cells (outline empty arrowhead), identified by anti-Foxd3 primary antibody (red), flank the neural plate after heat activation at the 3-somite stage. As shown in (B), transgenic embryos show significant loss of Foxd3 expression upon heat activation of hs-Tcf (green) as compared to with wild-type siblings (A). (C,D,E) Anti-Hu antibody, which recognizes the pan-neuronal marker Hu, was were used to identify Hu-positive cells in heat-activation treated embryos (red). Normal expression is detected in the trigeminal ganglia (white arrowhead), Rohon-Beard sensory neurons (white arrow), and primary motorneurons in the spinal column (asterisk) in Tcf embryos (D) as compared to with their wild-type siblings (C). Overlap between Hu and transgene expression is seen at higher power (E). All images are dorsal views of flat-mounted embryos fixed at the 10-somite stage, anterior to top, of stacked confocal series obtained at 10X (A-D) or 20X (E).