Fig. 5. Wnt8 and pax3 show overlapping expression in the presumptive neural crest domain. (A) Whole-mount in-situ hybridization at the 80% epiboly stage reveals expression of wnt8 transcripts at the margin and along the dorsal side of the embryo. (B) Expression of pax3 in the presumptive neural crest (arrow). (C) The wnt8 expression domain is in proximity to, and partially overlapping with, expression of pax3 in the presumptive neural crest region (black arrow). All images are lateral views, with dorsal to the right, of 80% epiboly stage embryos after hybridization.