Fig. 6. Wnt8 is required for neural crest induction in vivo. Whole-mount in-situ hybridization at the 6-somite stage reveals loss of early neural crest markers foxd3 (A-C), pax3 (D-F), and sox10 (G-I) upon injection of 20 mg/ml wnt8.1 Morpholino oligonucleotides (B,E,H). Such loss was not detected upon injection of 20 mg/ml wnt8.2 Morpholino (C,F,I), suggesting it does not have a role in neural crest induction. Uninjected controls (A,D,G) show normal expression of markers in the neural crest; foxd3 is also expressed in the notochord. All images are dorsal views, anterior to top, of 3-somite embryos.