Fig. 7. Functional Wnt8 is not required for late expression of neural crest markers. Whole-mount in-situ hybridization for neural crest marker, sox10, on 10-somite (A,B) and 24hpf (C,D) stage embryos. (E,F) Whole-mount in-situ hybridization for melanophore-specific marker, mitfa. (G,H) Whole-mount in-situ hybridization for cranial neural crest marker, dlx2. Injection of 20 mg/ml wnt8.1 antisense Morpholino oligonucleotides does not significantly reduce or eliminate the expression of any of these neural crest markers. All images of uninjected control siblings (A,C,E,G) and wnt8.1 MO-injected embryos (B,D,F,H) are lateral views (B-F) or dorsal views (A-B,G-H) with anterior to the left. Scale bar: 100 µm in (A,B); 200 µm in (C-F); 50 µm in (G,H).